Micro-Propagation Technologies (Theory & Practices + MCQs)

Master Plant Tissue Culture & Micro-Propagation with Theory, Practical Laboratory Protocols and MCQs — A Complete Guide for Agriculture, Horticulture & Plant Biotechnology Students

Micro-Propagation Technologies (Theory & Practices + MCQs) is a comprehensive resource designed for students, researchers, teachers and professionals working in agriculture, horticulture, plant biotechnology and plant tissue culture.

The book brings together theory, practical laboratory protocols and MCQs in a single volume. It takes the learner through the complete micro-propagation workflow, beginning with explant sterilization and media preparation and progressing through culture establishment, multiplication, rooting, regeneration, hardening and acclimatization.

Key Features

• Comprehensive coverage of plant tissue culture and micro-propagation technologies.
• Covers the complete micro-propagation workflow from explant sterilization to plant regeneration, hardening and acclimatization.
• Detailed treatment of organogenesis, callus culture and somatic embryogenesis.
• Includes seed and embryo culture, shoot-tip and meristem culture, bud culture and cell suspension cultures.
• Covers tissue-culture media preparation, stock solutions and culture conditions.
• Includes cryopreservation, somaclonal variation and secondary metabolite production.
• Dedicated section on commercial horticultural applications and case studies.
• Contains 15 practical laboratory protocols for hands-on learning.
• Includes practical procedures for MS nutrient media, autoclaving, sterilization, callus induction, embryo induction, regeneration and acclimatization.
• Includes troubleshooting of contamination and culture failure.
• Lesson-wise MCQs for learning, revision and examination preparation.
• Covers quality control, contamination management, ethical considerations and future trends

Table of Contents

Theory

• Introduction and history of micro-propagation
• Advantages and limitations of micro-propagation
• Seed and embryo culture
• Organ, callus and cell cultures
• Initiation and multiplication stages
• Rooting and acclimatization
• Axillary bud proliferation
• Shoot tip and meristem culture
• Bud culture for clonal propagation
• Organogenesis
• Callus culture
• Somatic embryogenesis
• Cell suspension cultures
• Secondary metabolite production
• Somaclonal variation
• Cryopreservation
• Explant sterilization
• Preparation of stock and working solutions
• Tissue-culture media and culture conditions
• Hardening of micropropagated plants
• Quality control and contamination management
• Commercial horticultural applications
• Case studies
• Future trends and innovations
• Ethical and environmental considerations

Practical Laboratory Section

The practical section contains 15 laboratory protocols, including:

• Tissue-culture laboratory equipment
• Preparation of Murashige and Skoog (MS) nutrient media
• Autoclaving of media and containers
• Sterilization of instruments and explants
• Preparation of stock solutions
• Preparation of working media
• Surface sterilization of seeds, shoot tips and nodes
• Culturing seeds and shoot tips
• Callus induction
• Somatic embryo induction
• Regeneration of whole plants
• Hardening and acclimatization
• Monitoring and documentation
• Troubleshooting contamination and culture failure
• Evaluation of plant regeneration and data analysis

MCQs

The book also includes MCQs according to the respective lesson plans, helping students evaluate their understanding and prepare for academic and competitive examinations.

Learning Outcomes

After studying this book, learners will be able to:

• Explain the principles, concepts and applications of micro-propagation and plant tissue culture.
• Understand the different stages of micro-propagation, from explant selection and sterilization to multiplication, rooting, regeneration and acclimatization.
• Explain the principles and applications of organogenesis, callus culture and somatic embryogenesis.
• Understand the preparation and use of plant tissue-culture media and stock solutions.
• Apply appropriate techniques for explant sterilization and contamination management.
• Understand practical laboratory procedures used in plant tissue culture and micro-propagation.
• Evaluate factors affecting plant regeneration, hardening and acclimatization.
• Understand applications of micro-propagation in commercial horticulture and plant biotechnology.
• Identify common laboratory problems and apply appropriate troubleshooting techniques.
• Use MCQs to assess, revise and strengthen their understanding for academic and competitive examinations.

Ideal For

This book is particularly useful for:

• B.Sc. Agriculture students
• M.Sc. Agriculture students
• Horticulture students
• Plant Biotechnology students
• Plant Tissue Culture students
• Agricultural researchers
• Teachers and extension professionals
• Students preparing for agriculture-related examinations
• Researchers working with in-vitro plant culture

Why Buy This Book?

• Combines theory, practical laboratory work and MCQs in one book.
• Provides a structured understanding of the complete micro-propagation process.
• Includes 15 practical laboratory protocols for hands-on learning.
• Covers important techniques such as organogenesis, callus culture and somatic embryogenesis.
• Helps students connect theoretical concepts with laboratory applications.
• Useful for both undergraduate and postgraduate students.
• Supports preparation for university, academic and competitive examinations through lesson-wise MCQs.
• Covers modern areas including cryopreservation, somaclonal variation, commercial applications and future trends.
• Useful as a reference for teachers, researchers and plant tissue-culture professionals

About the Authors

Professor (Dr.) A. Zaman is a Ph.D. in Agronomy with extensive experience in agricultural water management, irrigation, rainfed agriculture, cropping systems, watershed management and biodiversity conservation. He has held academic and research leadership positions and has authored numerous agricultural textbooks and research publications.

Dr. Md Hedayetullah is an Assistant Professor of Agronomy at Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal. His academic and research work includes agronomy, chickpea research, sustainable agriculture and agricultural development. He has authored/co-authored books and published research papers in agricultural sciences.